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Conserved domains on  [gi|383387816|ref|NP_001244299|]
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probable crossover junction endonuclease EME2 [Homo sapiens]

Protein Classification

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
XPF_nuclease_EME2 cd20082
XPF-like nuclease domain of crossover junction endonuclease EME2; EME2 interacts with MUS81 to ...
 68-260 2.37e-74

XPF-like nuclease domain of crossover junction endonuclease EME2; EME2 interacts with MUS81 to form a DNA structure-specific endonuclease which cleaves substrates such as 3'-flap structures. MUS81-EME2 is responsible for fork cleavage and restart in human cells. The MUS81-EME2 protein, whose actions are restricted to S phase, is also responsible for telomere maintenance in telomerase-negative ALT (Alternative Lengthening of Telomeres) cells. The nuclease domain of EME2 is a nuclease-like domain which is involved in targeting the MUS81-EME2 heterodimer complex to DNA.


:

Pssm-ID: 410858  Cd Length: 195  Bit Score: 229.14  E-value: 2.37e-74
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 383387816  68 RPEQVLKRLAVCVDTAILEDAGADVLMEALEALGCECRIEPQRPARSLRWTRASPDPCPRslpPEVWAAGEQELLLLLEP 147
Cdd:cd20082    1 RPEQCLKSLTVCVDPALLQDAGSDVLLEALSSLEWRYSIEPQSLPHSITWRRELPQDEPC---CEAGTVEEDQVLMVLEP 77

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 383387816 148 EEFLQGVATLTQ-------------ISGPTHWVPWISPETTArpHLAVIGLDAYLWSRQHVSRGTQQPespkvaGAEVAV 214
Cdd:cd20082   78 NEFLDMVASLKQvpngdgssgemesLLGPLFEYLNKDPTKVV--TLLVIGLDAYSWSNQLSGQKQKQL------GSELGM 149

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|....*.
gi 383387816 215 SWPEVEEALVLLQLWANLDVLLVASWQELSRHVCAVTKALAQYPLK 260
Cdd:cd20082  150 TDLDIEEALVFLQLHKNVSVLFLESWQELTDHVCAVTKALSKRPFK 195
 
Name Accession Description Interval E-value
XPF_nuclease_EME2 cd20082
XPF-like nuclease domain of crossover junction endonuclease EME2; EME2 interacts with MUS81 to ...
 68-260 2.37e-74

XPF-like nuclease domain of crossover junction endonuclease EME2; EME2 interacts with MUS81 to form a DNA structure-specific endonuclease which cleaves substrates such as 3'-flap structures. MUS81-EME2 is responsible for fork cleavage and restart in human cells. The MUS81-EME2 protein, whose actions are restricted to S phase, is also responsible for telomere maintenance in telomerase-negative ALT (Alternative Lengthening of Telomeres) cells. The nuclease domain of EME2 is a nuclease-like domain which is involved in targeting the MUS81-EME2 heterodimer complex to DNA.


Pssm-ID: 410858  Cd Length: 195  Bit Score: 229.14  E-value: 2.37e-74
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 383387816  68 RPEQVLKRLAVCVDTAILEDAGADVLMEALEALGCECRIEPQRPARSLRWTRASPDPCPRslpPEVWAAGEQELLLLLEP 147
Cdd:cd20082    1 RPEQCLKSLTVCVDPALLQDAGSDVLLEALSSLEWRYSIEPQSLPHSITWRRELPQDEPC---CEAGTVEEDQVLMVLEP 77

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 383387816 148 EEFLQGVATLTQ-------------ISGPTHWVPWISPETTArpHLAVIGLDAYLWSRQHVSRGTQQPespkvaGAEVAV 214
Cdd:cd20082   78 NEFLDMVASLKQvpngdgssgemesLLGPLFEYLNKDPTKVV--TLLVIGLDAYSWSNQLSGQKQKQL------GSELGM 149

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|....*.
gi 383387816 215 SWPEVEEALVLLQLWANLDVLLVASWQELSRHVCAVTKALAQYPLK 260
Cdd:cd20082  150 TDLDIEEALVFLQLHKNVSVLFLESWQELTDHVCAVTKALSKRPFK 195
 
Name Accession Description Interval E-value
XPF_nuclease_EME2 cd20082
XPF-like nuclease domain of crossover junction endonuclease EME2; EME2 interacts with MUS81 to ...
 68-260 2.37e-74

XPF-like nuclease domain of crossover junction endonuclease EME2; EME2 interacts with MUS81 to form a DNA structure-specific endonuclease which cleaves substrates such as 3'-flap structures. MUS81-EME2 is responsible for fork cleavage and restart in human cells. The MUS81-EME2 protein, whose actions are restricted to S phase, is also responsible for telomere maintenance in telomerase-negative ALT (Alternative Lengthening of Telomeres) cells. The nuclease domain of EME2 is a nuclease-like domain which is involved in targeting the MUS81-EME2 heterodimer complex to DNA.


Pssm-ID: 410858  Cd Length: 195  Bit Score: 229.14  E-value: 2.37e-74
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 383387816  68 RPEQVLKRLAVCVDTAILEDAGADVLMEALEALGCECRIEPQRPARSLRWTRASPDPCPRslpPEVWAAGEQELLLLLEP 147
Cdd:cd20082    1 RPEQCLKSLTVCVDPALLQDAGSDVLLEALSSLEWRYSIEPQSLPHSITWRRELPQDEPC---CEAGTVEEDQVLMVLEP 77

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 383387816 148 EEFLQGVATLTQ-------------ISGPTHWVPWISPETTArpHLAVIGLDAYLWSRQHVSRGTQQPespkvaGAEVAV 214
Cdd:cd20082   78 NEFLDMVASLKQvpngdgssgemesLLGPLFEYLNKDPTKVV--TLLVIGLDAYSWSNQLSGQKQKQL------GSELGM 149

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|....*.
gi 383387816 215 SWPEVEEALVLLQLWANLDVLLVASWQELSRHVCAVTKALAQYPLK 260
Cdd:cd20082  150 TDLDIEEALVFLQLHKNVSVLFLESWQELTDHVCAVTKALSKRPFK 195
XPF_nuclease_EME cd20083
XPF-like nuclease domain of crossover junction endonucleases, EME1, EME2 and similar proteins; ...
 76-260 8.90e-37

XPF-like nuclease domain of crossover junction endonucleases, EME1, EME2 and similar proteins; The Mus81-EME1 complex is a structure-selective endonuclease with a critical role in the resolution of recombination intermediates during DNA repair after interstrand cross-links, replication fork collapse, or double-strand breaks. ERCC4 domain of Eme1 is a nuclease-like domain which is involved in targeting the MUS81-EME1 heterodimer complex to DNA.


Pssm-ID: 410859  Cd Length: 179  Bit Score: 131.64  E-value: 8.90e-37
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 383387816  76 LAVCVDTAILEDAGADVLMEALEALGCECRIEPQRPARSLRWTRASPDPCPR--SLPPEVWAAGEQELLLLLEPEEFLQG 153
Cdd:cd20083    2 IRVVIDPKILEESYGGELLSALQEKGLKYEIESQPIPNSITWTRNVPEDTVAdnEVALEESEEDEPYVLLILSAEEFVKM 81

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 383387816 154 VATLTqISGPTHWVPWISPEttARPHLAVIGLDAYLWSRQHVSRGTQqpespkVAGAEVAVSWPEVEEALVLLQLWANLD 233
Cdd:cd20083   82 VKNGT-LLDHISSVKSLFPN--YPITLVIYGLNKYKRYHKKKEQSKK------KKKNLKNVSRPPVEEALIELQLHAKCS 152

                        170       180
                 ....*....|....*....|....*..
gi 383387816 234 VLLVASWQELSRHVCAVTKALAQYPLK 260
Cdd:cd20083  153 SRLCETEAELALHVAQLTKAIAEAPYK 179
XPF_nuclease_EME1 cd20081
XPF-like nuclease domain of crossover junction endonuclease EME1; EME1, also called MMS4 ...
 68-263 4.58e-20

XPF-like nuclease domain of crossover junction endonuclease EME1; EME1, also called MMS4 homolog (hMMS4), interacts with MUS81 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Its typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. EME1 may be required in mitosis for the processing of stalled or collapsed replication forks. The nuclease domain of EME1 is a nuclease-like domain which is involved in targeting the MUS81-EME1 heterodimer complex to DNA.


Pssm-ID: 410857  Cd Length: 179  Bit Score: 86.73  E-value: 4.58e-20
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 383387816  68 RPEQVLKRLAVCVDTAILEDAGADVLMEALEALGCECRIEPQRPARSLRWTRASPDPCPRS------------LPPEVWA 135
Cdd:cd20081    1 RPEECLKHIVVVLDPGLLQMEGGGQLLSALQAMECSCVIEAQAIPRSVTWRRRAAPSQVDEedwveeptvlvlLPAEEFV 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 383387816 136 AgeqelllllEPEEFLQGVATLTQISGPT--HWVPWISPETTAR-PHLAVIGLDAYLwsrqhvsrgtqqpespkvagaev 212
Cdd:cd20081   81 S---------MVHNYKQESLGSTTEGKETlqSFVTDITAKTAGKaLSLVVVDMEKYF----------------------- 128

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|.
gi 383387816 213 AVSWPEVEEALVLLQLWANLDVLLVASWQELSRHVCAVTKALAQYPLKQYR 263
Cdd:cd20081  129 RVSRVDVEEALVDLQLHTGVQVQFLETWKEFADFICMFTKAVAEAPFKKLR 179
XPF_nuclease_EME-like cd20080
XPF-like nuclease domain of the family of Essential Meiotic Endonucleases (EMEs) and similar ...
 218-260 6.03e-03

XPF-like nuclease domain of the family of Essential Meiotic Endonucleases (EMEs) and similar proteins; The family of EMEs includes EME1 and EME2. EME1, also called MMS4 homolog (hMMS4), interacts with MUS81 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Its typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. EME1 may be required in mitosis for the processing of stalled or collapsed replication forks. EME2 interacts with MUS81 to form a DNA structure-specific endonuclease which cleaves substrates such as 3'-flap structures. MUS81-EME2 is responsible for fork cleavage and restart in human cells. The MUS81-EME2 protein, whose actions are restricted to S phase, is also responsible for telomere maintenance in telomerase-negative ALT (Alternative Lengthening of Telomeres) cells. The nuclease domain of EMEs is a nuclease-like domain which is involved in targeting the MUS81-EME heterodimer complex to DNA. The family also includes budding yeast Mms4 (also known as Eme1 in other organisms), a putative transcriptional (co)activator that protects Saccharomyces cerevisiae cells from endogenous and environmental DNA damage. It interacts with MUS81 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. The nuclease domain of Mms4 lacks the catalytic motif.


Pssm-ID: 410856  Cd Length: 164  Bit Score: 37.37  E-value: 6.03e-03
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|...
gi 383387816 218 EVEEALVLLQLWANLDVLLVASWQELSRHVCAVTKALAQYPLK 260
Cdd:cd20080  122 DAEEALVDLQLHTEAQAQIVQSWKELADFTCAFTKAVAEAPFK 164
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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