GEO DataSets

(Submitter supplied) In multiple myeloma (MM), abnormal plasma cells interact with bone marrow (BM) stromal cells and vascular cells among others. A part of the BM milieu is considered highly hypoxic, and myeloma cells in situ may be influenced by circumstances other than normoxia in vitro. Hence, we attempted to confirm the role of hypoxic MM-derived exosomes in the BM milieu. We established a novel hypoxia-resistant cell line, KMS-11-HR, derived from KMS-11 cells cultured for >4 months under hypoxia (1% O2), as a model of MM cells localizing in an extensively hypoxic milieu. more...

Organism:

Homo sapiens

Type:

Expression profiling by RT-PCR

Platform:

GPL13987

6 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens

Type:

Expression profiling by RT-PCR

Platform:

GPL13987

18 Samples

Download data

(Submitter supplied) In multiple myeloma (MM), abnormal plasma cells interact with bone marrow (BM) stromal cells and vascular cells among others. A part of the BM milieu is considered highly hypoxic, and myeloma cells in situ may be influenced by circumstances other than normoxia in vitro. Hence, we attempted to confirm the role of hypoxic MM-derived exosomes in the BM milieu. We established a novel hypoxia-resistant cell line, U266HR, derived from U266 cells cultured for >4 months under hypoxia (1% O2), as a model of MM cells localizing in an extensively hypoxic milieu. more...

Organism:

Homo sapiens

Type:

Expression profiling by RT-PCR

Platform:

GPL13987

6 Samples

Download data: TXT

(Submitter supplied) In multiple myeloma (MM), abnormal plasma cells interact with bone marrow (BM) stromal cells and vascular cells among others. A part of the BM milieu is considered highly hypoxic, and myeloma cells in situ may be influenced by circumstances other than normoxia in vitro. Hence, we attempted to confirm the role of hypoxic MM-derived exosomes in the BM milieu. We established a novel hypoxia-resistant cell line, RPMI8226HR, derived from RPMI8226 cells cultured for >4 months under hypoxia (1% O2), as a model of MM cells localizing in an extensively hypoxic milieu. more...

Organism:

Homo sapiens

Type:

Expression profiling by RT-PCR

Platform:

GPL13987

6 Samples

Download data: TXT

(Submitter supplied) MicroRNAs (miRNAs) regulate a wide range of cellular signaling pathways and biological processes in both physiological and pathological states such as cancer. We have previously identified miR-135b as a direct regulator of androgen receptor (AR) protein level in prostate cancer (PCa). We wanted to further explore the relationship of miR-135b to hormonal receptors, particularly estrogen receptor α (ERα). more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS6100

Platform:

GPL10558

12 Samples

Download data: TXT

Analysis of LNCaP prostate cancer (PCa) cells overexpressing miRNA-135b for up to 36 hours. LNCaP cells express the androgen receptor (AR). MiRNA-135b overexpression in AR+ PCa cells results in slower growth compared to AR knockdown. Results provide insight into the basis of this slower growth.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 2 protocol, 3 time sets

Platform:

GPL10558

Series:

GSE57820

12 Samples

Download data

(Submitter supplied) Recently, the existence of extracellular miRNAs enclosed in exosomes has raised the possibility that they play an important role in cell-cell communication. To gain more insight into cell-cell communication via exosomal miRNAs, we investigated whether or not tumor cells exposed to hypoxia secrete exosomes which may affect angiogeneic activity. We used RPMI8226 cells, as donor cells, and HUVECs as recipient cells. more...

Organism:

Homo sapiens

Type:

Other

Platform:

GPL13987

4 Samples

Download data: TXT

(Submitter supplied) Recently, the existence of extracellular miRNAs enclosed in exosomes has raised the possibility that they play an important role in cell-cell communication. To gain more insight into cell-cell communication via exosomal miRNAs, we investigated whether or not tumor cells exposed to hypoxia secrete exosomes which may affect angiogeneic activity. We used SUDHL4 cells, as donor cells, and HUVECs as recipient cells. more...

Organism:

Homo sapiens

Type:

Other

Platform:

GPL13987

4 Samples

Download data: TXT

(Submitter supplied) Recently, the existence of extracellular miRNAs enclosed in exosomes has raised the possibility that they play an important role in cell-cell communication. To gain more insight into cell-cell communication via exosomal miRNAs, we investigated whether or not tumor cells exposed to hypoxia secrete exosomes which may affect angiogeneic activity. We used K562 cells, as donor cells, and HUVECs as recipient cells. more...

Organism:

Homo sapiens

Type:

Other

Platform:

GPL8979

4 Samples

Download data: TXT

(Submitter supplied) we analyzed transcriptomic profiles in LLC cells isolated from primary cancer sites. C57BL/6 mice were subcutaneously injected with LLC-RFP with or without BMSCs.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23479

6 Samples

Download data: TXT

(Submitter supplied) Exosomal miRNAs including 86 exosomal miRNAs were significantly increased and 354 exosomal miRNAs were significantly decreased in the co-injection group compared to LLC injection alone through expression profiling of a total of 1903 genes.

Organism:

Mus musculus

Type:

Non-coding RNA profiling by array

Platform:

GPL21265

2 Samples

Download data: TXT

(Submitter supplied) MiRNA microarray analysis was performed on exosomes secreted by mouse MSC cells under two different conditions of normal oxygen and hypoxia, in order to find out the different miRNAs in exosomes secreted by MSC under two different conditions.

Organism:

Mus musculus

Type:

Non-coding RNA profiling by array

Platform:

GPL22383

2 Samples

Download data: TXT

(Submitter supplied) How cancer cells adapt to hypoxia during tumor development remains an important question. The hypothesis tested in the present study was that tumor cell-derived exosome vesicles (also known as microvesicles or extracellular vesicles) are mediators of hypoxia-dependent intercellular signaling in glioblastoma (GBM), i.e. highly aggressive brain tumors characterized by hypoxia and a vascular density that is among the highest of all human malignancies. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6947

12 Samples

Download data: TXT

(Submitter supplied) qPCR-RT pre-designed plate to study miRNAs expression in urinary exosome from LN patients before and after treatment. We have included 14 LN patients. Of them, there is 7 LN patients with clinical response and 7 with no clinical response.

Organism:

Homo sapiens

Type:

Expression profiling by RT-PCR

Platform:

GPL27772

26 Samples

Download data: CSV, TXT

(Submitter supplied) We conducted whole miRNA screening against the MM patients bone marrow (sorted by CD38++) cells that were subjected by chronic hypoxia condition.

Organism:

Homo sapiens

Type:

Non-coding RNA profiling by array

Platform:

GPL18402

8 Samples

Download data: TXT

(Submitter supplied) By using world wildly used myeloma cell lines (RPMI8226, KMS-11, KMS-12BM, and MM.1S), we conducted whole miRNA screening against the cells that were subjected by chronic hypoxia condition.

Organism:

Homo sapiens

Type:

Non-coding RNA profiling by array

Platform:

GPL18402

8 Samples

Download data: TXT

(Submitter supplied) We conducted whole genome gene screening against the MM patients bone marrow (sorted by CD38++) cells that were subjected by chronic hypoxia condition.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL17077

8 Samples

Download data: TXT

(Submitter supplied) By using myeloma cell lines (RPMI8226, KMS-11, KMS-12BM, and MM.1S), we conducted whole genome gene screening against the cells that were subjected by chronic hypoxia condition.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL17077

8 Samples

Download data: TXT

(Submitter supplied) In multiple myeloma (MM), hypoxia-inducible transcription factor-1 (HIF-1) is overexpressed in the MM cells of the hypoxic bone marrow (BM) microenvironment. Herein, we explored in MM cells the in vitro and in vivo effects of persistent HIF-1 inhibition by expression of a lentivirus shRNA pool on proliferation, survival and transcriptional and pro-angiogenic profiles. Among the significantly modulated genes (326 and 361 genes in hypoxic and normoxic condition, respectively), we found that HIF-1 inhibition in the human myeloma cell line JJN3 downregulates the pro-angiogenic molecules VEGF, IL8, IL10, CCL2, CCL5, and MMP9. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL15445

4 Samples

Download data: CEL

(Submitter supplied) MicroRNAs (miRNAs), small (18–22 nucleotides) non-coding RNAs, suppress the translation of target mRNAs by binding to their 3′ untranslated region. Evidence suggests that miRNAs are key regulators of several cellular processes, including angiogenesis. Recent findings indicate that Secreted miRNAs enclosed in exosomes also play an important role in cell–cell communication. To elucidate whether miRNAs secreted from neoplastic cells transfer into endothelial cells and are functionally active in the recipient cells, we investigated the interaction of a leukemia cell line and human umbilical vein endothelial cells (HUVECs).

Organism:

Homo sapiens

Type:

Non-coding RNA profiling by array

Platform:

GPL11487

2 Samples

Download data: TXT