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Inhibition of full-length human DNMT2 expressed in bacteria using tRNA-Asp as substrate and [3H[-SAM as cosubstrate at 100 uM by tritium incorporation assay
Assay data:8 Tested
SummaryPubMed CitationRelated BioAssays by Target
Inhibition of human DNMT2 by tritium incorporation assay
Assay data:1 Tested
Binding affinity to human DNMT2 deletion mutant expressed in Escherichia coli Rosetta 2 (DE3) pLysS cells incubated for 1 hr under denaturing condition by protein mass spectrometry
Assay data:2 Tested
Inhibition of full-length human DNMT2 expressed in bacteria in presence of DTT by tritium incorporation assay
Inhibition of full-length human DNMT2 expressed in bacteria using tRNA-Asp as substrate and [3H[-SAM as cosubstrate at 100 uM by tritium incorporation assay relative to control
Assay data:29 Tested
Binding affinity to full-length human DNMT2 expressed in bacteria assessed as apparent dissociation constant by ITC method
Assay data:5 Active, 1 Activity ≤ 1 µM, 29 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
Displacement of FTAD from full-length human DNMT2 expressed in bacteria incubated for 10 mins by MST assay
Assay data:4 Active, 29 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
Displacement of FTAD from full-length human DNMT2 expressed in Escherichia coli incubated for 10 mins by MST assay
Displacement of FTAD from full-length human DNMT2 expressed in bacteria at 20 uM incubated for 10 mins by MST assay relative to control
Assay data:30 Tested
Binding affinity to human DNMT2 at 100 uM by tritium incorporation assay relative to control
Inhibition of full-length human DNMT2 expressed in bacteria using tRNA-Asp as substrate and 3H-SAM as cosubstrate by tritium incorporation assay
Assay data:5 Active, 29 Tested
Inhibition of DNMT2-mediated tRNA methylation in human HEK293 cells assessed as reduction in m5C at C38 of tRNA by measuring m5C level at 100 uM incubated for 24 hrs by bisulfite sequencing analysis
Inhibition of DNMT2-mediated tRNA methylation in human HEK293 cells assessed as reduction in m5C at C38 of tRNA at 100 uM incubated for 24 hrs by LC-MS/MS analysis
Inhibition of full length human N-terminal his6-tagged DNMT2 expressed in Escherichia coli Rosetta2(DE3)pLysS at 100 uM by liquid scintillation counting analysis
Assay data:6 Tested
Binding affinity to full length human N-terminal his6-tagged DNMT2 expressed in Escherichia coli Rosetta2(DE3)pLysS assessed as change in entropy at 100 uM by isothermal titration calorimetry assay
Binding affinity to full length human N-terminal his6-tagged DNMT2 expressed in Escherichia coli Rosetta2(DE3)pLysS assessed as change in enthalpy change at 100 uM by isothermal titration calorimetry assay
Binding affinity to full length human N-terminal his6-tagged DNMT2 expressed in Escherichia coli Rosetta2(DE3)pLysS assessed as gibbs free energy change at 100 uM by isothermal titration calorimetry assay
Inhibition of full length human N-terminal his6-tagged DNMT2 expressed in Escherichia coli Rosetta2(DE3)pLysS by isothermal titration calorimetry assay
Binding affinity to full length human N-terminal his6-tagged DNMT2 expressed in Escherichia coli Rosetta2(DE3)pLysS assessed as dissociation constant at 100 uM by isothermal titration calorimetry assay
Assay data:2 Active, 6 Tested
Inhibition of full length human N-terminal his6-tagged DNMT2 expressed in Escherichia coli Rosetta2(DE3)pLysS at 100 uM using tRNA asp as substrate and SAM as co-substrate incubated for 5 mins by tritium incorporation assay relative to control
Assay data:38 Tested
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