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| Status |
Public on Jun 26, 2024 |
| Title |
SoxB1 transcription factors are essential for intiating and maintaining neural plate border gene expression [ChIP-seq] |
| Organism |
Xenopus laevis |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Here we investigate a functional role for SoxB1 transcription factors, Sox2 and Sox3, on the transient embryonic cell population, the neural plate border. We find through gain/loss-of-function studies necessary for neural plate border cells to become neural crest cells. and genomics experiments (ChIP-seq and RNA-seq) that SoxB1 transcription factors directly promote neural plate border formation. and that down-regulation of SoxB1 expression is
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| Overall design |
We identify regions of Sox3 enrichment in blastula stem cells and neural plate border cells (St 11.5) through ChIP-seq studies. Furthermore, we assess changes in gene expression in soxb1 morphant (vs WT) blastula stem cells through RNA-seq.
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| Contributor(s) |
Schock EN, York JR, Li AP, Tu AY, LaBonne C |
| Citation(s) |
38940470 |
| |
| Submission date |
Jan 05, 2024 |
| Last update date |
Oct 01, 2024 |
| Contact name |
Carole LaBonne |
| Organization name |
Northwestern University
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| Department |
Molecular Biosciences
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| Street address |
2220 Campus Drive
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| City |
Evanston |
| State/province |
IL |
| ZIP/Postal code |
60208 |
| Country |
USA |
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| Platforms (2) |
| GPL21248 |
Illumina NextSeq 500 (Xenopus laevis) |
| GPL28901 |
Illumina NovaSeq 6000 (Xenopus laevis) |
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| Samples (12)
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| This SubSeries is part of SuperSeries: |
| GSE252617 |
SoxB1 transcription factors are essential for intiating and maintaining neural plate border gene expression |
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| Relations |
| BioProject |
PRJNA1061523 |
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