|
| Status |
Public on Jan 01, 2012 |
| Title |
The transcription factor nurr1 exerts concentration-dependent effects on target genes mediating distinct biological processes |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
The transcription factor nurr1 plays a pivotal role in the development and maintenance of neurotransmitter phenotype in midbrain dopamine neurons. Conversely, decreased nurr1 expression is associated with a number of dopamine-related CNS disorders, including Parkinson’s disease and drug addiction. In order to better understand the nature of nurr1-responsive genes and their potential roles in dopamine neuron differentiation and survival, we used a neural cellular background in which to generate a number of stable clonal lines with graded nurr1 gene expression that approximated that seen in DA cell-rich human substantia nigra. Gene expression profiling data from these nurr1-expressing clonal lines were validated by quantitative RT-PCR and subjected to bioinformatic analyses. The present study identified a large number of nurr1-responsive genes and demonstrated the potential importance of concentration-dependent nurr1 effects in the differential regulation of distinct nurr1 target genes and biological pathways. These data support the promise of nurr1-based CNS therapeutics for the neuroprotection and/or functional restoration of DA neurons.
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| |
|
| Overall design |
Total RNA obtained from nurr1-overexpressing SKNAS neuroblastoma clonal cell lines (SKNAS_E & SKNAS_G) compared to empty vector transfected control (SKNAS_C)
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| |
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| Contributor(s) |
Johnson MM, Michelhaugh SK, Bouhamdan M, Schmidt CJ, Bannon MJ |
| Citation(s) |
22194714 |
| |
| Submission date |
Nov 03, 2011 |
| Last update date |
Aug 16, 2018 |
| Contact name |
Magen Marie Johnson |
| E-mail(s) |
mmdavis@med.wayne.edu
|
| Organization name |
Wayne State University
|
| Department |
Pharmacology
|
| Lab |
Bannon
|
| Street address |
540 E Canfield
|
| City |
Detroit |
| State/province |
MI |
| ZIP/Postal code |
48201 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL6947 |
Illumina HumanHT-12 V3.0 expression beadchip |
|
| Samples (9)
|
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| Relations |
| BioProject |
PRJNA148685 |
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