|
| Status |
Public on Oct 28, 2012 |
| Title |
PBMC C3 IL1 -2h 6334175048_B |
| Sample type |
RNA |
| |
|
| Source name |
PBMC
|
| Organism |
Homo sapiens |
| Characteristics |
disease state: Healthy
subject: Healthy 3
stimulation: IL-1b
time: 2 hours
cell type: PBMC
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using the RNeasy Mini Kit (Qiagen) according to the manufacturer's instructions
|
| Label |
biotin
|
| Label protocol |
Biotynlated cRNA were generated following manufacturer protocols using the Illumina TotalPrep RNA Amplification Kit from Ambion
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol and scanned with Illumina iScan
|
| Data processing |
The data were background subtracted and average normalized using Illumina Genome Studio
|
| |
|
| Submission date |
Sep 12, 2012 |
| Last update date |
Oct 28, 2012 |
| Contact name |
Zhaohui Xu |
| Organization name |
St. Jude Children's Research Hospital
|
| Department |
Infectious Diseases
|
| Street address |
262 Danny Thomas Place
|
| City |
Memphis |
| State/province |
TN |
| ZIP/Postal code |
38105 |
| Country |
USA |
| |
|
| Platform ID |
GPL10558 |
| Series (2) |
| GSE40752 |
Transcriptional analysis of whole blood, primary fibroblasts, and PBMCs upon TNF-alpha or IL-1beta stimulation from HOIL-1-deficient patients |
| GSE40838 |
Transcriptome analysis in peripheral blood mononuclear cells (PBMC) from HOIL-1-deficient patients upon TNF-α or IL-1β stimulation |
|
