|
| Status |
Public on Dec 01, 2012 |
| Title |
Patient 7 - Repeat Biopsy |
| Sample type |
RNA |
| |
|
| Source name |
Renal needle biopsy
|
| Organism |
Homo sapiens |
| Characteristics |
progression: Repeat
activity: high
chronicity: high
years to dialysis: --
tissue: Renal needle biopsy
disease state: Lupus Nephritis
subject: Patient 7
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from FFPE kidney specimens using RecoverAll Total RNA Isolation Kit (Ambion Inc, TX). RNA qualities were assessed with NanoDrop 2000 to determine the concentration and 260/280 ratio.
|
| Label |
biotin
|
| Label protocol |
Asuragen has a proprietary labeling method.
|
| |
|
| Hybridization protocol |
Performed by Asuragen using Affymetrix GeneChip miRNA Array protocols.
|
| Scan protocol |
Performed by Asuragen using Affymetrix GeneChip miRNA Array protocols.
|
| Data processing |
RMA Background correction with quantile normalization.
|
| |
|
| Submission date |
Nov 30, 2012 |
| Last update date |
Dec 01, 2012 |
| Contact name |
Ilias Alevizos |
| E-mail(s) |
alevizosi@mail.nih.gov
|
| Organization name |
National Institute for Dental and Craniofacial Research (NIDCR)
|
| Lab |
Sjogren's Syndrome Clinic
|
| Street address |
10 Center Dr., RM 1N110
|
| City |
Bethesda |
| State/province |
Maryland |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL8786 |
| Series (1) |
| GSE42648 |
miRNA profiling of repeat kidney biopsies in Lupus Nephritis |
|
