|
| Status |
Public on Sep 01, 2013 |
| Title |
siLRH-1_#2_rep_1 |
| Sample type |
RNA |
| |
|
| Source name |
MCF-7 human cell line, siLRH-1_#2
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: MCF-7
sirna knockdown: siLRH-1_#2
|
| Treatment protocol |
Cells were transfected with double-stranded RNA oligonucleotides using the Lipofectamine RNAiMax reverse transfection method (Invitrogen, UK), according to manufacturer's protocols. Two different siRNAs against LRH-1 were used: ON-TARGETplus siRNA J-003430-07 (siLRH-1_#2) and J-003430-08 (siLRH-1_#3) from Dharmacon. siGENOME Non-targeting siRNA (Dharmacon, cat. no.:D-001210-01) (siControl) was used as negative control. All siRNA experiments used the double-stranded RNA oligonucleotides at a final concentration of 80 nM.
|
| Growth protocol |
MCF-7 cells were cultured in DMEM containing 10% FCS.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the RNeasy Kit from QIAGEN following manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion Illumina® TotalPrep™-96 RNA Amplification Kit for Illumina arrays.
|
| |
|
| Hybridization protocol |
Labelled cRNA was hybridised to the Illumina HumanHT-12 V3.0 expression BeadChip according to the Illumina Whole-Genome Gene Expression Direct Hybridization Assay Guide.
|
| Scan protocol |
BeadChips were scanned using the BeadArray reader.
|
| Description |
SAMPLE 5
Replicate 1 of 4.
|
| Data processing |
The BeadChiP image data were preprocessed using GenomeStudio (Illumina). The expression data were then log2 transformed and quantile-normalised using Partek Genomics Suite.
|
| |
|
| Submission date |
Jun 10, 2013 |
| Last update date |
Sep 01, 2013 |
| Contact name |
Simak Ali |
| E-mail(s) |
simak.ali@imperial.ac.uk
|
| Organization name |
Imperial College London
|
| Department |
Department of Surgery & Cancer
|
| Street address |
Du Cane Road
|
| City |
London |
| ZIP/Postal code |
W12 0NN |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL6947 |
| Series (1) |
| GSE47803 |
Co-regulated gene expression by estrogen receptor-α and liver receptor homolog-1 is a feature of the estrogen response in breast cancer cells |
|
