|
| Status |
Public on Dec 03, 2013 |
| Title |
VCaP cells treated for 24 hours with R1881, experiment 2 |
| Sample type |
RNA |
| |
|
| Source name |
VCaP
|
| Organism |
Homo sapiens |
| Characteristics |
treatment: R1881
date of experiment: 10.05.2010
timepoint: 24
|
| Treatment protocol |
Cells were cultured in the absence (EtOH) or presence of 1nM R1881 for 8 and 24 hours, respectively.
|
| Growth protocol |
Cells (LNCaP, VCaP, DuCaP) were grown on polylysine-coated (Sigma) petridishes in RPMI1640 supplemented with 10% charcoal-stripped FCS overnight.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using TRI-reagent (Sigma) according to the manufacturer`s protocol. After DNA digestion with DnaseI (Qiagen), RNA was purified using Rneasy kit (Qiagen).
|
| Label |
biotin
|
| Label protocol |
Target RNA was labeled by addition of biotin-linked deoxynucleotides with terminal deoxynucleotidyl transferase according to the manufacturer's (Affymetrix) protocols. Washing and staining was performed in an Affymetrix 450S fluidics station.
|
| |
|
| Hybridization protocol |
Target samples were hybridized to Human Gene 1.0 ST v1 microarrays according to the manufacturer's protocols in a Affymetrix hybridization oven.
|
| Scan protocol |
Microarrays were scanned in an Affymetrix 3000 scanner.
|
| Description |
VCaP cells treated for 24 hours with R1881, experiment 2
|
| Data processing |
Raw data was preprocessed using the GCRMA method. For transcript probe set definition a custom CDF package based on a complete re-alignment of the probe sequences to the genome and transcriptome (Ensembl version 68) was used; details on http://bioinfo.i-med.ac.at.
|
| |
|
| Submission date |
Sep 17, 2013 |
| Last update date |
Dec 04, 2013 |
| Contact name |
Johannes Rainer |
| E-mail(s) |
johannes.rainer@eurac.edu
|
| Organization name |
Eurac Researc
|
| Department |
Institute for Biomedicine
|
| Lab |
Biomedical Informatics
|
| Street address |
Via A. Volta 21
|
| City |
Bolzano |
| ZIP/Postal code |
39100 |
| Country |
Italy |
| |
|
| Platform ID |
GPL17737 |
| Series (1) |
| GSE50936 |
SOCS2 expression correlates with tumor malignancy, exerts growth promoting effects and is enhanced by androgens in prostate cancer |
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