|
| Status |
Public on Dec 27, 2013 |
| Title |
RNA_HVH_sample78 [HumanHT-12 V3.0] |
| Sample type |
RNA |
| |
|
| Source name |
RNA_HVH
|
| Organism |
Homo sapiens |
| Characteristics |
age (yrs): 70
gender: female
tissue: blood
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using PAXgene Blood RNA Kit (QIAGEN Inc., Valencia, CA)
|
| Label |
biotin
|
| Label protocol |
Total RNA labeled with biotin using Illumina® TotalPrep™-96 RNA Amplification Kit (Life Technologies Corp., Carlsbad, CA)
|
| |
|
| Hybridization protocol |
Total RNA was hybridized onto Illumina HumanHT-12v3 Beadchip arrays (Illumina, San Diego, CA)
|
| Scan protocol |
Scanned images of the array chips were imported into Illumina’s GenomeStudio Gene Expression Module
|
| Data processing |
quantile normalization using R version 2.15.2 and the package "aroma.light". Normalized results were then log2 transformed.
|
| |
|
| Submission date |
Dec 26, 2013 |
| Last update date |
Dec 27, 2013 |
| Contact name |
Daniel A Enquobahrie |
| Organization name |
University of Washington
|
| Department |
Cardiovascular Health Research Unit
|
| Street address |
1730 Minor Avenue
|
| City |
Seattle |
| State/province |
Washington |
| ZIP/Postal code |
98101 |
| Country |
USA |
| |
|
| Platform ID |
GPL6947 |
| Series (2) |
| GSE47727 |
Global peripheral blood gene expression study [HumanHT-12 V3.0] |
| GSE47729 |
Global peripheral blood gene expression study |
|
