|
| Status |
Public on Nov 30, 2010 |
| Title |
Liver_EA (agilent) |
| Sample type |
RNA |
| |
|
| Source name |
Liver total RNA, EA stage
|
| Organism |
Urocitellus parryii |
| Characteristics |
tissue: liver
stage: early arousal
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from each tissue sample was extracted using TRizol (Invitrogen) according to the manufacturer's instructions. Total RNA quality was assessed by Bioanalyzer 2100 (Agilent). Total RNA of arctic ground squirrels from the same stage were mixed in the equal amount into three pooled samples: EA, LT, and PR.100ng total RNA from three pooled samples was used as input in the labeling reaction.
|
| Label |
Cy3
|
| Label protocol |
Labeling and hybridization of total RNA were performed according to the manufacturer’s protocol with no modification.
|
| |
|
| Hybridization protocol |
Same as above. The entire reaction was hybridized to the array for 20 hours at 55°C.
|
| Scan protocol |
Microarray results were extracted using Agilent Microarray Scanner (Agilent)
|
| Description |
18-30nt RNA in EA stage
|
| Data processing |
The miRNA signals and errors on miRNA microarray were obtained from gTotalGeneSignal and gTotalGeneError respectively in the output files of Agilent Feature Extraction software (v9.5.3.1), subsequently analyzed by Gene-Spring GX 7.3.1 software (Agilent).
|
| |
|
| Submission date |
Dec 11, 2009 |
| Last update date |
Nov 30, 2010 |
| Contact name |
Wenchao Hu |
| Organization name |
CAS-MPG Partner Institute for Computational Biology
|
| Department |
Functional Genomics Group
|
| Street address |
320 Yueyang Road
|
| City |
Shanghai |
| ZIP/Postal code |
200031 |
| Country |
China |
| |
|
| Platform ID |
GPL7732 |
| Series (2) |
| GSE19427 |
miRNA profiling by Agilent mouse miRNA microarray |
| GSE19808 |
miRNA profiling of miRNAs in Hibernating Arctic Ground Squirrels |
|
