|
| Status |
Public on Nov 15, 2012 |
| Title |
Diabetic female adipose 7 |
| Sample type |
RNA |
| |
|
| Source name |
Subcutaneous adipose
|
| Organism |
Homo sapiens |
| Characteristics |
gender: Female
age: 48
tissue: Subcutaneous adipose
disease state: diabetic
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Biopsy samples were stored in RNAlater (Ambion) in minus 80 degree C before RNA was extracted. Total cellular RNA was extracted using mirVana isolation kit (Ambion). The quantity and the quality of RNA samples were determined using Nanodrop-1000 (Thermo Fischer Scientific, Wilmington, USA) and Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA), in that order.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNAs were prepared using Illumina TotalPrep kit (Ambion).
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol was followed.
|
| Scan protocol |
Standard Illumina scanning protocol was followed.
|
| Description |
Diabetic_Biological replicate _1_technical_replicate_2
|
| Data processing |
Non-normalized data was obtained by subtracting background from raw intensities in GenomeStudio data analysis software (Illumina). The data were normalized using average normalization. The non-diabetic group was selected as the reference, Illumina custom method was used as the error model, and Benjamini and Hochberg False Discovery Rate was used as the method for multiple testing correction.
|
| |
|
| Submission date |
May 11, 2011 |
| Last update date |
Nov 15, 2012 |
| Contact name |
Abhay Sharma |
| E-mail(s) |
abhaysharma@igib.res.in
|
| Phone |
+91-11-27666156
|
| Fax |
+91-11-27667602
|
| Organization name |
CSIR-Institute of Genomics and Integrative Biology (IGIB)
|
| Department |
Functional Genomics Unit
|
| Street address |
Delhi University campus, Mall Road
|
| City |
Delhi |
| State/province |
Delhi |
| ZIP/Postal code |
110007 |
| Country |
India |
| |
|
| Platform ID |
GPL6947 |
| Series (1) |
| GSE29226 |
Global Gene Expression Profiles of Subcutaneous Adipose in Females with Type 2 Diabetes. |
|
