|
| Status |
Public on Nov 04, 2013 |
| Title |
MB1 TREG |
| Sample type |
RNA |
| |
|
| Source name |
MB1 TREG
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Tregs (CD4+ CD25+ CD127lo)
non pregnant, cord blood, or maternal blood: maternal blood
|
| Treatment protocol |
cells sorted by flow cytometry and added to cells to signal lysis buffer and stored at minus 80 degrees celcius until RNA extraction
Whole blood samples collected using PAXgene RNA tubes and stored at -20 degrees Celcius until processing
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using Qiagen Rneasy mini kit
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion TotalPrep RNA Amplification Kit kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
5125398042_B
|
| Data processing |
Data was normalised using quantile normalisation algorithm in BRB arrayTools
|
| |
|
| Submission date |
Sep 08, 2011 |
| Last update date |
Nov 04, 2013 |
| Contact name |
Grant Parnell |
| E-mail(s) |
gpar3109@uni.sydney.edu.au
|
| Organization name |
University of Sydney
|
| Department |
Nepean Clinical School
|
| Lab |
Intensive Care
|
| Street address |
Nepean Hospital
|
| City |
Kingswood |
| State/province |
NSW |
| ZIP/Postal code |
2747 |
| Country |
Australia |
| |
|
| Platform ID |
GPL6947 |
| Series (1) |
| GSE31976 |
regulatory T cells in human pregnancy |
|
