|
| Status |
Public on Oct 28, 2012 |
| Title |
P3 IL1 - 2h (6334175027_G) |
| Sample type |
RNA |
| |
|
| Source name |
fibroblast
|
| Organism |
Homo sapiens |
| Characteristics |
disease state: HOIL deficiency
subject: HOIL deficiency 3
stimulation: IL-1b
time: 2 hours
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using the MagMAX™-96 Blood RNA Isolation Kit (Applied Biosystems) according to the manufacturer's instructions
|
| Label |
biotin
|
| Label protocol |
Biotynlated cRNA were generated following manufacturer protocols using the Illumina TotalPrep RNA Amplification Kit from Ambion.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
We used Illumina iScan.
|
| Description |
P3 IL1 - 2h
|
| Data processing |
The data were analysed using BeadStudio version using the average chip normalization method.
|
| |
|
| Submission date |
Sep 03, 2012 |
| Last update date |
Oct 28, 2012 |
| Contact name |
Zhaohui Xu |
| Organization name |
St. Jude Children's Research Hospital
|
| Department |
Infectious Diseases
|
| Street address |
262 Danny Thomas Place
|
| City |
Memphis |
| State/province |
TN |
| ZIP/Postal code |
38105 |
| Country |
USA |
| |
|
| Platform ID |
GPL10558 |
| Series (2) |
| GSE40560 |
Transcriptome analysis in primary fibroblasts from HOIL-1-deficient patients upon TNF-α or IL-1β stimulation |
| GSE40752 |
Transcriptional analysis of whole blood, primary fibroblasts, and PBMCs upon TNF-alpha or IL-1beta stimulation from HOIL-1-deficient patients |
|
