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| Status |
Public on Feb 24, 2013 |
| Title |
Induction of ER stress in the colon cancer cell line LS174 with SubAB |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
To assess the effect of activation of the unfolded protein response (UPR) in colon cancer cell lines, we treated cells with the AB5 subtilase cytotoxin (SubAB). This proteolytically cleaves the 78-kDa glucose-regulated protein (GRP78; also known as HSPA5 or BiP) inside the endoplasmic reticulum. We find that the WNT signaling pathway is highly affected upon treatment with SubAB.
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| Overall design |
500,000 cells were plated in a 10 cm2 plate, and the following day, treated for 20 hrs with SubAB or the control (SubAA272B), and then harvested. Three independent biological replicates per treatment.
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| Contributor(s) |
Heijmans J, van den Brink GR |
| Citation(s) |
23545496, 27819675 |
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| Submission date |
Apr 07, 2011 |
| Last update date |
Jul 20, 2021 |
| Contact name |
Jarom Heijmans |
| E-mail(s) |
j.heijmans@amc.nl
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| Organization name |
Academic Medical Center
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| Department |
Tytgat Institute for liver and intestinal research
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| Lab |
van den Brink Group
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| Street address |
Meibergdreef 69-71
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| City |
Amsterdam |
| ZIP/Postal code |
1105 BK |
| Country |
Netherlands |
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| Platforms (1) |
| GPL6947 |
Illumina HumanHT-12 V3.0 expression beadchip |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA143225 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE28466_RAW.tar |
6.2 Mb |
(http)(custom) |
TAR |
| Processed data not provided for this record |
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