|
| Status |
Public on Feb 24, 2013 |
| Title |
LS174, control, rep3 |
| Sample type |
RNA |
| |
|
| Source name |
colon cancer cell line LS174, control
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: LS174
cell type: colon cancer cells
treatment: SubAA272B
|
| Treatment protocol |
100 ng ml-1 SubAB or control SubAA272B for 20 hours.
|
| Growth protocol |
DMEM 10% FCS penicillin/streptomycin.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol, subsequently Qiagen Rneasy cleanup.
|
| Label |
Biotin
|
| Label protocol |
Ambion cRNA synthesis for Illumina.
|
| |
|
| Hybridization protocol |
According to Illumina instructions.
|
| Scan protocol |
According to Illumina instructions on the BeadArray 500GX scanner.
|
| Description |
Replicate 3 of 3.
|
| Data processing |
Data was extracted in GenomeStudio. No normalization was performed.
|
| |
|
| Submission date |
Apr 07, 2011 |
| Last update date |
Feb 24, 2013 |
| Contact name |
Jarom Heijmans |
| E-mail(s) |
j.heijmans@amc.nl
|
| Organization name |
Academic Medical Center
|
| Department |
Tytgat Institute for liver and intestinal research
|
| Lab |
van den Brink Group
|
| Street address |
Meibergdreef 69-71
|
| City |
Amsterdam |
| ZIP/Postal code |
1105 BK |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL6947 |
| Series (1) |
| GSE28466 |
Induction of ER stress in the colon cancer cell line LS174 with SubAB |
|
