|
| Status |
Public on Jan 01, 2015 |
| Title |
LBL_severly affected_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Lymphoblastoid cell line
|
| Organism |
Homo sapiens |
| Characteristics |
gender: female
sma: SMAI
smn2 copies: 2
experiemental group: 2
sample: B9994
|
| Treatment protocol |
no treatment
|
| Growth protocol |
lyphoblastoid cell lines grown in RPMI 1640 Medium without L.Glutamine
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA extraction with TRIzol (Invitrogen)
|
| Label |
biotin
|
| Label protocol |
400 ng of total RNA were linearly amplified and biotinylated using the Illumina® TotalPrepTM RNA Amplification Kits (Ambion) according to the manufacturer’s instructions
|
| |
|
| Hybridization protocol |
Human HT-12v3 bead arrays (Illumina, San Diego, CA) were hybridized with 750ng cRNA for 18h at 58°C according to the Illumina® Whole-Genome Gene Expression with IntelliHyb Seal System Manual
|
| Scan protocol |
Arrays were washed three times with buffer E1BC, High-Temp Wash Buffer and 100% ethanol, respectively, stained with streptavidine-Cy3 and again washed with buffer E1BC
|
| Description |
E9R_034b02
|
| Data processing |
Raw fluorescence intensities were recorded on a BeadArray Reader GX (Illumina). Average signal intensities without background correction (Dunning et al., 2008) were performed with BeadStudio 3.1 software (Illumina). Arrays were normalized with VSN (Bioconductor version 2.6.1) including a ln transformation step
|
| |
|
| Submission date |
Jun 09, 2014 |
| Last update date |
Jan 01, 2015 |
| Contact name |
Michael H. Walter |
| E-mail(s) |
michael.walter@agilent.com
|
| Organization name |
University of Tuebingen
|
| Department |
Department of Medical Genetics
|
| Lab |
The Microarray Facility Tübingen
|
| Street address |
Calwer Str. 7
|
| City |
Tuebingen |
| ZIP/Postal code |
72076 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6947 |
| Series (1) |
| GSE58316 |
Suppression of NCALD protects against spinal muscular atrophy by restoring impaired endocytosis |
|
